Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23.

@article{Lin1998ProductionAP,
  title={Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23.},
  author={L L Lin and Charng-cherng Chyau and Winston H. Hsu},
  journal={Biotechnology and applied biochemistry},
  year={1998},
  volume={28 1},
  pages={
          61-8
        }
}
The optimum temperature and initial medium pH for amylase production by Bacillus sp. TS-23 were 55 degrees C and 8.5 respectively. Maximum amylase activity was obtained in a medium containing peptone and soluble starch as nitrogen and carbon sources. Activity staining revealed that two amylases with molecular masses of 150 and 42 kDa were produced when maltose, soluble starch or amylose was used as carbon source for growth, whereas only the 150 kDa protein was detected in the medium containing… CONTINUE READING

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