Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein.

@article{Nakamura2008ProductionAH,
  title={Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein.},
  author={Kouichi C Nakamura and Hiroshi Kameda and Yoshinori Koshimizu and Yuchio Yanagawa and Takeshi Kaneko},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2008},
  volume={56 7},
  pages={647-57}
}
Enhanced green fluorescent protein (GFP) irreversibly loses not only fluorescence but also antigenicity recognized with conventional anti-GFP antibodies by heat denaturation. This hinders combinatory applications of the GFP immunodetection technique with heat-requiring procedures, such as in situ hybridization histochemistry, antigen retrieval, and Western blot. Here we produced new rabbit and guinea pig antibodies against heat-denatured GFP. The polyclonal antibodies affinity-purified with the… CONTINUE READING