Production and comprehensive quality control of recombinant human Interleukin-1beta: a case study for a process development strategy.

@article{Block2008ProductionAC,
  title={Production and comprehensive quality control of recombinant human Interleukin-1beta: a case study for a process development strategy.},
  author={Helena Block and Jan Kubicek and J{\"o}rg Labahn and Udo Roth and Frank Schaefer},
  journal={Protein expression and purification},
  year={2008},
  volume={57 2},
  pages={244-54}
}
We describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL-1beta). The protein preparation was apparently free of… CONTINUE READING