Production and characterization of single-chain antibody (scFv) against 3ABC non-structural protein in Escherichia coli for sero-diagnosis of Foot and Mouth Disease virus.

@article{Sharma2014ProductionAC,
  title={Production and characterization of single-chain antibody (scFv) against 3ABC non-structural protein in Escherichia coli for sero-diagnosis of Foot and Mouth Disease virus.},
  author={Gaurav Kumar Sharma and Sonalika Mahajan and Rakesh Matura and Saravanan Subramaniam and Jajati Keshari Mohapatra and Bramhadev Pattnaik},
  journal={Biologicals : journal of the International Association of Biological Standardization},
  year={2014},
  volume={42 6},
  pages={339-45}
}
Differentiation of Foot-and-Mouth Disease infected from vaccinated animals is essential for effective implementation of vaccination based control programme. Detection of antibodies against 3ABC non-structural protein of FMD virus by immunodiagnostic assays provides reliable indication of FMD infection. Sero-monitoring of FMD in the large country like India is a big task where thousands of serum samples are annually screened. Currently, monoclonal or polyclonal antibodies are widely used in… CONTINUE READING
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