Production and characterization of monoclonal antibodies to bovid herpesvirus-4.

  title={Production and characterization of monoclonal antibodies to bovid herpesvirus-4.},
  author={Jean Dubuisson and {\'E}tienne Thiry and Michel Bublot and M Sneyers and Denise S. M. Boulanger and J. -C. Guillaume and P. P. Pastoret},
  journal={Veterinary microbiology},
  volume={19 4},
Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a… CONTINUE READING



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