Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1.

@article{Azorsa1999ProductionAC,
  title={Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1.},
  author={David O Azorsa and Paul S. Meltzer},
  journal={Hybridoma},
  year={1999},
  volume={18 3},
  pages={281-7}
}
The steroid coactivator AIB1 (Ampified In Breast cancer 1) is a member of the SRC-1 family of transactivation coactivators that is amplified in about 7% of breast tumor samples. Hybridomas were established that secreted monoclonal antibodies (MAbs) to a recombinant glutathione-S-transferase (GST) fusion protein expressing the steroid receptor interacting domain of AIB1 (GST-AIB.T1:a.a. 605-1294). Four MAbs from these hybridomas were characterized and designated AX15.1, AX15.2, AX15.3, and AX15… CONTINUE READING