Production and characterization of monoclonal antibodies against rabbit growth cartilage

@article{Okihana1990ProductionAC,
  title={Production and characterization of monoclonal antibodies against rabbit growth cartilage},
  author={Hiroyuki Okihana and Yutaka Shimomura},
  journal={International Orthopaedics},
  year={1990},
  volume={14},
  pages={321-327}
}
SummaryGrowth cartilage (GC) cells of young rabbits were cultured in vitro and their homogenates were injected into mice. Hybridomas were prepared by the cell fusion technique between the myeloma cells and the spleen cells of the immunized mice. Monoclonal antibodies (MoAbs) were produced by the hybridomas in the peritoneal cavities of the mice, and some of these, temporarily named MoAbs A, B, D, N, P, and S, were studied. The localization of the antigens of each of the MoAbs in the GC or… CONTINUE READING
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