Production and characterization of monoclonal antibodies against ochratoxin B.

@article{Heussner2007ProductionAC,
  title={Production and characterization of monoclonal antibodies against ochratoxin B.},
  author={Alexandra H. Heussner and Ines Moeller and Billy W. Day and Daniel R Dietrich and Evelyn O'brien},
  journal={Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association},
  year={2007},
  volume={45 5},
  pages={827-33}
}
Monoclonal antibodies against ochratoxin B (OTB) were generated by immunizing Balb/c mice with OTB conjugated to keyhole limpet hemocyanin (KLH) via carbodiimide reactions with CHMC and EDAC. A stable hybridoma cell line 2F1.E10 was produced by fusion of murine splenocytes and myeloma cells. The obtained antibodies were characterized using an indirect competitive ELISA. The detection limit was calculated (27+/-2 nM OTB) and 50% binding inhibition was reached at 500 nM free OTB. A low cross… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 37 references

Production of hybridomas

  • D. Baron
  • 1992
Highly Influential
5 Excerpts

Development of a sensitive enzyme-linked immunosorbent assay for the determination of ochratoxin A

  • Yu, F.-Y, +5 authors C.-C
  • J. Agric. Food Chem
  • 2005
Highly Influential
6 Excerpts

Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay

  • Cho, Y.-J, +10 authors J.-H
  • J. Agric. Food Chem
  • 2005
Highly Influential
6 Excerpts

Selection and cloning

  • A. M. Campbell
  • 1991
Highly Influential
2 Excerpts

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