Production and characterization of heat-aggregated IgG complexes with pre-determined molecular masses: light-scattering study.

@article{Ostreko1987ProductionAC,
  title={Production and characterization of heat-aggregated IgG complexes with pre-determined molecular masses: light-scattering study.},
  author={K K Ostreĭko and I A Tumanova and Sykulev YuK},
  journal={Immunology letters},
  year={1987},
  volume={15 4},
  pages={311-6}
}
A method for the preparation of model immune complexes of heat-aggregated human IgG with predetermined molecular masses is described. IgG complexes with different molecular masses were produced by incubation of human IgG for 20 min at 63 degrees C, the protein concentration varying from 0.5 to 5 mg/ml before heat treatment. To determine the bulk of IgG molecules included in the aggregates, the IgG complexes obtained were precipitated by 7% polyethylene glycol. The relative molecular masses of… CONTINUE READING

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