Production and characterization of biodegradable terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) by Alcaligenes sp. A-04.

  title={Production and characterization of biodegradable terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) by Alcaligenes sp. A-04.},
  author={Suchada Chanprateep and Songsri Kulpreecha},
  journal={Journal of bioscience and bioengineering},
  volume={101 1},
The production of the terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate), P(3HB-co-3HV-co-4HB), by Alcaligenes sp. A-04 was investigated to determine the superior biodegradable polymer properties over those of poly(3-hydroxybutyrate), P(3HB), and its copolymers. The highest terpolymer content of 68% (w/w) was produced by Alcaligenes sp. A-04 at 60 h by shake-flask cultivation. The terpolymer with 93 mol% 4HB mole fraction units was produced when the cultivation time… 

Tables from this paper

Biosynthesis and Characterization of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) Terpolymer with Various Monomer Compositions by Cupriavidus sp. USMAA2-4

The monomer compositions of 3HV and 4HB were found to have great influences on the thermal and mechanical properties of the terpolymer P(3HB-co-3HV- co-4HB) produced.

Improvement of the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) terpolyester by manipulating the culture condition

The combination of oleic acid with I³-butyrolactone and 1-pentanol was found to be the best combination to produce high PHA content (81 wt%) and this manipulated culture condition can be used at larger scale to provide modeling for the production of terpolyester in a bioreactor.

Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379

The aim of this work was to maximize the Polyhydroxyalkanoates (PHA) yields with a high proportion of 3HV and 4HB using different culture strategies.

Studies on the Microbial Synthesis and Characterization of Polyhydroxyalkanoates Containing 4-Hydroxyvalerate Using γ-Valerolactone

In this study, the ability of Cupriavidus sp. USMAA2-4 to synthesize polyhydroxyalkanoates (PHA) containing 4-hydroxyvalerate monomer (4HV) was studied through one-stage cultivation using

Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Cupriavidus necator B-10646 from Mixtures of Oleic Acid and 3-Hydroxyvalerate Precursors

This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors, and can be used to develop and implement an economically feasible process of the P( 3HB- co-3 HV) production.

Production and characterization of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) co‐polymer by a N2‐fixing cyanobacterium, Nostoc muscorum Agardh

The material properties of the films were found to be comparable with that of the commercial polymer, thus advocating its potential applications in various fields.

Properties of PHA bi-, ter-, and quarter-polymers containing 4-hydroxybutyrate monomer units.

A fed-batch strategy to produce high poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) terpolymer yield with enhanced mechanical properties in bioreactor

The fed-batch fermentation strategies employed in this study have resulted in terpolymers with a range of flexible materials having improved tensile strength and Young’s modulus as compared to the terpolymer produced from batch fermentation.



Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans.

  • Y. SaitoY. Doi
  • Biology, Materials Science
    International journal of biological macromolecules
  • 1994
The biodegradabilities of P(3HB-co-4HB) films were studied in aqueous solutions of extracellular polyhydroxybutyrate (PHB) depolymerase from Alcaligenes faecalis or of lipase from Rhizopus delemer, and the erosion rate of films by lipase increased with the 4HB fraction.

Nonisothermal Crystallization Kinetics of Biodegradable Random Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Block One

The kinetics of nonisothermal crystallization in a novel microbial synthesis of a biodegradable block copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), in which the mole fractions

Identification of 4-hydroxyhexanoic acid as a new constituent of biosynthetic polyhydroxyalkanoic acids from bacteria

A terpolyester consisting of 3-hydroxybutyric acid (3HB), 3-Hydroxyhexanoic acid ( 3HHx) and 4HHx was synthesized, as revealed by gas chromatographic analysis of the accumulated polyester and as confirmed by nuclear magnetic resonance spectroscopicAnalysis of the isolated polyester.

Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic

An overview of the different PHA biosynthetic systems and their genetic background is provided, followed by a detailed summation of how this natural diversity is being used to develop commercially attractive, recombinant processes for the large-scale production of PHAs.

Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation

  • J. ChoiS. Lee
  • Environmental Science
    Applied Microbiology and Biotechnology
  • 1999
Abstract Polyhydroxyalkanoates (PHA) have been attracting considerable attention as biodegradable substitutes for conventional polymers. To reduce their production cost, a great deal of effort has

Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography

A convenient gas-liquid chromatography procedure to quantify poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge was developed by combining lyophilization of the samples,