Production and characterization of amphihaploid hybrids between Nicotiana wuttkei Clarkson et Symon and N. tabacum L

  title={Production and characterization of amphihaploid hybrids between Nicotiana wuttkei Clarkson et Symon and N. tabacum L},
  author={Dorota Laskowska and Apoloniusz Berbe{\'c}},
Nicotiana wuttkei Clarkson and Symon discovered in the 1990s in Australia may be of potential interest to breeders as it carries resistance to Peronospora hyoscyami de Bary. The crossability between N. wuttkei (2n = 4x = 32) and three N. tabacum (2n = 4x = 48) cultivars (‘Puławski 66’, ‘Wiślica’ and ‘TN 90’) and the morphology and cytology of their amphihaploid hybrids (2n = 4x = 40) were studied. Seeds were produced only when N. wuttkei was used as the maternal parent, but under normal… 

Figures and Tables from this paper

Cytology and fertility of amphidiploid hybrids between Nicotiana wuttkei Clarkson et Symon and N. tabacum L.
In this study, the morphology, cytology and crossability of amphidiploid hybrids (2n = 8x = 80; WWW′W′TTT′T′) between Nicotiana wuttkei × Nicotianatabacum were studied. An R1 progeny of the hybrids
Species Origin of Genomic Factors in Nicotiana nudicaulis Watson Controlling Hybrid Lethality in Interspecific Hybrids between N. nudicaulis Watson and N. tabacum L
It is speculated that when N. nudicaulis was formed approximately 5 million years ago, several causative genomic factors determining phenotypes of hybrid seedlings were inherited from N. trigonophylla and some became genomic factor(s) controlling hybrid lethality in extant N. tabacum.
The Use of Nicotiana Species in Tobacco Improvement
This review examines how and to what extent natural germplasm resources have been utilized in hybridization and introgression experiments over the past century, and focuses on individual Nicotiana species involved in interspecific hybrids with cultivated tobacco produced by sexual and asexual methods.
Hybrid Lethality in the Genus Nicotiana
This chapter reviews studies of hybrid lethality in the genus Nicotiana and describes several terms used to describe these phenomena, i.e., Hybrid lethality, hybrid weakness, hybrid necrosis and hybrid inviability.
Effectiveness of combining va-mediated resistance with a lettuce mosaic virus coat protein gene and a potato virus Y polymerase RNA gene in protection of tobacco hybrids from Potato virus Y
The most effective combination of va factor and transgene LMV CP was observed for VAM x MN 944 LMV and Wiślica x MN 900 LMV because a complete resistance was maintained in subsequent generations in the case of these hybrids.


Q Chromosome Controls the Lethality of Interspecific Hybrids between Nicotiana tabacum and N. suaveolens
Results suggested that the Q chromosome of the S genome harbored a gene or genes that induced non-viable seedlings from a cross between N. tabacum and N. suaveolens, and revealed that 3 viable plants were true F1 hybrids.
Bypassing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization
In vitro pollination of placenta attached ovules was useful in bypassing unilateral incongruity barriers for several Nicotiana interspecific hybrid combinations (N. amplexicaulis, N. benthamiana, and ‘Ky 17’ X N. repanda) and it appears that it may be possible to obtain asexual hybrids of N. arentsii or N. bonariensis with N. tabacum.
Genomic factors controlling the lethality exhibited in the hybrid between Nicotiana suaveolens Lehm. and N. tabacum L.
Findings suggest that the S genome in N. tabacum is responsible for the lethality exhibited in the hybrid between N. suaveolens and N.tabacum, and could be avoided by culturing the ovules at 36 °C.
Cytogenetic studies in Nicotialea XVIII. Reduction divisions in three interspecific hybrids between autotetraploid N. tabacum and three other species
The reduction division in PMC's was studied in 3 interspecific hybrids : 4x N. tabacum X N. Ialegsdorffii and 4X N. beleevidesii into cultivated tobacco, where the purpose of producing this hybrid was to introduce a gene or genes for immunity to mildew and for resistance to black root rot from N. alata.
Preliminary study of the newly discovered tobacco species Nicotiana wuttkei Clarkson et Symon
Plant morphology and chromosome pairing data suggest that N. wuttkei has a closer affinity to N. maritima than toN.
Overcoming hybrid incompatibility between Nicotiana africana Merxm. and N. tabacum and development of cytoplasmically male sterile tobacco forms
The incompatibility between the wild species N. africana Merxm. and the cultivated species N. tabacum has been overcome by in vitro techniques. Underdeveloped F0 seeds, placed on MS medium with
Seven of eight species in Nicotiana section Suaveolentes have common factors leading to hybrid lethality in crosses with Nicotiana tabacum.
Hybrid lethality was observed in all crosses at 28 degrees C, and was Type II lethality, with the characteristic symptoms of browning of hypocotyl and roots, suggesting that many species of section Suaveolentes share the same factor that triggers hybrid lethality by interaction with the genes on the Q chromosome.
Meiotic analysis of haploid and doubled haploid forms of Nicotiana otophora and N. tabacum
There is very little intra- or intergenomic pairing, if any, in Nicotiana haploids studied, and the meiotic stability of the doubled haploids permits using these materials directly in the breeding program.
Disease resistant flue-cured tobacco breeding lines for north Queensland I. Resistance to blue mould, Peronospora tabacina
Five backcross lines, three double haploid lines and one line carrying resistance from two sources were selected for further screening as potential commercial cultivars for blue mould resistance and for commercial attributes.
Molecular systematics, GISH and the origin of hybrid taxa in Nicotiana (Solanaceae).
Relying solely upon ITS nrDNA analysis to reveal phylogenetic patterns in a complex genus such as Nicotiana is insufficient, and it is clear that conventional analysis of single data sets, such as ITS, is likely to be misleading in at least some respects about evolutionary history.