Production and characterization of a specific 5-HT2 receptor antibody.

  title={Production and characterization of a specific 5-HT2 receptor antibody.},
  author={Steven J. Garlow and David A Morilak and Raymond S. Dean and Bryan L. Roth and Roland D. Ciaranello},
  journal={Brain research},
  volume={615 1},
A synthetic peptide was used to generate antibodies against the rat serotonin-2 (5-HT2) receptor. The peptide corresponds to a unique sequence from the N-terminal extracellular portion of the receptor protein (antibody = Ab 5HT2-N). This peptide was chosen based on its theoretical antigenic index and for specificity to the 5-HT2 receptor. In dot blot analysis, antisera detected 2 ng-2 micrograms of synthetic peptide at dilutions of 1/200-1/20,000. COS-7 cells transiently transfected with a… CONTINUE READING


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