Production and characterization of a recombinant beta-1,4-endoglucanase (glycohydrolase family 9) from the termite Reticulitermes flavipes.

@article{Zhou2010ProductionAC,
  title={Production and characterization of a recombinant beta-1,4-endoglucanase (glycohydrolase family 9) from the termite Reticulitermes flavipes.},
  author={X. Zhou and E. Kovaleva and D. Wu-Scharf and James H Campbell and G. Buchman and D. Boucias and M. Scharf},
  journal={Archives of insect biochemistry and physiology},
  year={2010},
  volume={74 3},
  pages={
          147-62
        }
}
Cell-1 is a host-derived beta-1,4-endoglucanase (Glycohydrolase Family 9 [GHF9]) from the lower termite Reticulitermes flavipes. Here, we report on the heterologous production of Cell-1 using eukaryotic (Baculovirus Expression Vector System; BEVS) and prokaryotic (E. coli) expression systems. The BEVS-expressed enzyme was more readily obtained in solubilized form and more active than the E. coli-expressed enzyme. K(m) and V(max) values for BEVS-expressed Cell-1 against the model substrate CMC… Expand
Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris
TLDR
RsEGm could be overexpressed in P. pastoris and may have potential to be used in the field of laundry, textile and lignocellulose-based biofuels and chemicals. Expand
Functional and translational analyses of a beta-glucosidase gene (glycosyl hydrolase family 1) isolated from the gut of the lower termite Reticulitermes flavipes.
TLDR
Findings suggest that RfBGluc-1 may serve dual functions in cellulose digestion and immunity, and contributes to the development of next-generation termiticides and novel biocatalyst cocktails for use in biomass-to-bioethanol applications. Expand
cDNA cloning and heterologous expression of an endo-β-1,4-glucanase from the fungus-growing termite Macrotermes barneyi.
TLDR
The cDNA cloning of another endogenous cellulase, the EG protein MbEG1, indicated that it could be functionally expressed in P. pastoris and the information that may facilitate understanding of cellulolytic systems in fungus-growing termites is provided. Expand
Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4
TLDR
It is confirmed that extracellular cellulolytic capability of E. coli ZH-4 was attributed to BcsZ, which explained why E. bacteria can grow on cellulose and has some new characteristics which will extend the understanding of endo-glucanase. Expand
Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi.
TLDR
The goal of this work was to decipher the mode of operation of CgEG1 and CgBG1 through a comprehensive biochemical analysis and molecular docking studies and give insight about the role of these two enzymes in termite biology, which can provide the foundation for the development of a number of important applied research topics. Expand
A GHF7 cellulase from the protist symbiont community of Reticulitermes flavipes enables more efficient lignocellulose processing by host enzymes.
TLDR
The hypothesis that protist GHF7 cellulases are capable of synergistic interactions with host termite digestive enzymes is supported only in the case of GHF3, and the findings suggest that not all protist cellulases will enhance saccharification by cocktails of other termite or fungal lignocellulases. Expand
Functional characterization and target discovery of glycoside hydrolases from the digestome of the lower termite Coptotermes gestroi
TLDR
This is the first global study on the enzymatic repertoire involved in plant polysaccharide degradation by the lower termite C. gestroi, and the biochemical characterization of whole body termite extracts evidenced their ability to cleave all types of glycosidic bonds present in plantpolysaccharides. Expand
Carbohydrate‐active enzymes revealed in Coptotermes formosanus (Isoptera: Rhinotermitidae) transcriptome
TLDR
The normalized EST library provides a comprehensive genetic resource of C. formosanus and will serve a diverse range of research areas, and represents one of the repositories of enzymes useful for physiological studies and applications in sugar‐based biofuel production. Expand
Functional analyses of the digestive β-glucosidase of Formosan subterranean termites (Coptotermes formosanus).
TLDR
It is highlighted that the β-glucosidase is one of the critical cellulases responsible for cellulose degradation and glucose production; inactivation of these digestive enzymes by specific inhibitors may starve the termite. Expand
Molecular Cloning of Five &bgr;-Glucosidases from Four Species of Higher Termites (Blattodea: Termitidae)
TLDR
Five amino acid sequences obtained from four species of higher termites with differing nutritional biologies indicated that they were most similar to &bgr;-glucosidases in the glycosyl hydrolase family 1 (GHF1), and all complete coding sequences are 490 amino acids long. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 36 REFERENCES
Heterologous Expression and Characterization of an Endoglucanase from a Symbiotic Protist of the Lower Termite, Reticulitermes speratus
TLDR
Thin-layer chromatography analysis revealed that RsSymEG1 preferentially hydrolyzes the β-1,4-cellulosic linkage of cellodextrins into cellobiose and glucose. Expand
New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites
TLDR
An endo-β-1,4-glucanase was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus, and showed high homology to EGs from glycoside hydrolase family (GHF) 7. Expand
Functional and translational analyses of a beta-glucosidase gene (glycosyl hydrolase family 1) isolated from the gut of the lower termite Reticulitermes flavipes.
TLDR
Findings suggest that RfBGluc-1 may serve dual functions in cellulose digestion and immunity, and contributes to the development of next-generation termiticides and novel biocatalyst cocktails for use in biomass-to-bioethanol applications. Expand
Molecular cloning and characterization of a cellulase gene from a symbiotic protist of the lower termite, Coptotermes formosanus.
The endo-beta-1,4-glucanase gene was cloned from a cDNA library constructed from the mixed population of symbiotic protists in the hindgut of the lower termite, Coptotermes formosanus, using theExpand
Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from Coptotermes formosanus and expressed in E. coli.
TLDR
The results presented in this report uncovered the biochemical properties of the recombinant cellulase derived from the intact gene of Formosan subterranean termites, useful in designing cellulase-inhibiting termiticides and incorporating into a sugar-based biofuel production program. Expand
Heterologous Overexpression of a Mutant Termite Cellulase Gene in Escherichia coli by DNA Shuffling of Four Orthologous Parental cDNAs
TLDR
A chimeric library by family shuffling of endo-β-1,4-glucanase genes from four different termite species was constructed and a mutant enzyme showed high activity against CMC and properties similar to those of the native enzymes. Expand
cDNA cloning, expression, and enzymatic activity of a cellulase from the mulberry longicorn beetle, Apriona germari.
  • S. Lee, S. R. Kim, +7 authors B. Jin
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2004
TLDR
The Ag-EGase enzyme assay exhibited high activity only in midgut tissue, suggesting that the midGut is the prime site where large quantities of EGase are synthesized for degrading the absorbed cellulose from the diet. Expand
Characterization of a functional soluble form of a Brassica napus membrane-anchored endo-1,4-beta-glucanase heterologously expressed in Pichia pastoris.
TLDR
The functional analysis of Delta(1-90)Cel16, the N terminally truncated Cel16, missing residues 1 through 90 and comprising the catalytic domain of Cel16 expressed recombinantly in the methylotrophic yeast Pichia pastoris as a soluble protein, showed that Delta( 1-90)-hydrolyzed carboxymethylcellulose is a true endo-acting glucanase. Expand
Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari.
  • Y. Wei, K. Lee, +6 authors B. Jin
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2006
TLDR
A novel endogenous beta-1,4-endoglucanase (Ag-EGase III) gene belonging to the glycoside hydrolase family (GHF) 5 was cloned from the mulberry longicorn beetle, Apriona germari, indicating that the foregut and midgut are the prime sites for cellulase synthesis in A. germari larvae. Expand
A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity.
  • S. Lee, K. Lee, +7 authors B. Jin
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2005
TLDR
Midgut-specific expression of Ag-EGase II is confirmed, suggesting that the midgut is the prime site for cellulase synthesis in A. germari larvae, and the enzymatic properties of the purified recombinant Ag- EGase II showed the highest activity at 50 degrees C and pH 6.0. Expand
...
1
2
3
4
...