Production and characterization of a monoclonal antibody cross-reactive with most group A trichothecenes.

@article{Fan1988ProductionAC,
  title={Production and characterization of a monoclonal antibody cross-reactive with most group A trichothecenes.},
  author={T S Fan and Susan L Schubring and Ru-dong Wei and Fun Sun Chu},
  journal={Applied and environmental microbiology},
  year={1988},
  volume={54 12},
  pages={2959-63}
}
A monoclonal antibody cross-reactive with most group A trichothecenes was produced by fusion of P3/NS-1/1-AG4-1 myeloma cells with spleen cells isolated from a BALB/c mouse that had been immunized with 3-acetyl-neosolaniol-hemisuccinate conjugated to bovine serum albumin. One stable clone, H159B1D5, which produced monoclonal antibody that bound with both T-2 toxin and diacetoxyscirpenol (DAS) was obtained after subcloning. Enzyme-linked immunosorbent assay (ELISA) revealed that the antibody… CONTINUE READING

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