Production and characterization of a camelid single domain antibody-urease enzyme conjugate for the treatment of cancer.

@article{Tian2015ProductionAC,
  title={Production and characterization of a camelid single domain antibody-urease enzyme conjugate for the treatment of cancer.},
  author={Baomin Tian and Wah Yau Wong and Elda Hegmann and K. J. Gaspar and Praveen Kumar and Heman Chao},
  journal={Bioconjugate chemistry},
  year={2015},
  volume={26 6},
  pages={
          1144-55
        }
}
A novel immunoconjugate (L-DOS47) was developed and characterized as a therapeutic agent for tumors expressing CEACAM6. The single domain antibody AFAIKL2, which targets CEACAM6, was expressed in the Escherichia coli BL21 (DE3) pT7-7 system. High purity urease (HPU) was extracted and purified from Jack bean meal. AFAIKL2 was activated using N-succinimidyl [4-iodoacetyl] aminobenzoate (SIAB) as the cross-linker and then conjugated to urease. The activation and conjugation reactions were… 
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21 Citations

Development and Characterization of a Camelid Single Domain Antibody–Urease Conjugate That Targets Vascular Endothelial Growth Factor Receptor 2
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Convenient and Universal Fabrication Method for Antibody-Enzyme Complexes as Sensing Elements Using the SpyCatcher/SpyTag System.
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This method results in the convenient and universal preparation of sensing elements with the potential for electrochemical measurement and confirmed the universality of this AEC fabricating method by applying it to another VHH.
Novel single-domain antibodies against the EGFR domain III epitope exhibit the anti-tumor effect
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Anticancer Activity of Urease Mimetic Cobalt (III) Complexes on A549-Lung Cancer Cells: Targeting the Acidic Microenvironment
TLDR
The study confirms the urease mimicking anticancer activity of cobalt (III) complexes by neutralizing the tumour microenvironment.
Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing
TLDR
The methods for fabricating AECs using fragment antibodies developed for sensing applications are summarized and the advantages and disadvantages of each method are discussed.
Improving the targeting of therapeutics with single-domain antibodies
TLDR
This work has shown that the identification of novel protein modification techniques will provide more options for sdAb modification (conjugation, immobilization, functionalization), allowing a wider array of therapeutic agents to be successfully targeted and delivered using sdAbs.
Rapid and homogeneous electrochemical detection by fabricating a high affinity bispecific antibody-enzyme complex using two Catcher/Tag systems.
Single-Domain Antibodies as Therapeutic and Imaging Agents for the Treatment of CNS Diseases
TLDR
The characteristic properties of single-domain antibodies compared to traditional antibodies are introduced and recent advances in the development of sdAbs are presented, including their use for the delivery of biologics across the blood–brain and blood–cerebrospinal fluid barriers, treatment of neurodegenerative diseases and molecular imaging of brain targets.
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TLDR
This review provides a comprehensive overview on the state-of-the-art regarding the use of nanobodies as theranostics, and their importance in the emerging field of personalized medicine.
Acidosis and cancer: from mechanism to neutralization
TLDR
This review will revisit the causes and consequences of acidosis by summarizing strategies used by cancer cells to adapt to acidosis, and how neutralization of acidity can be used to inhibit carcinogenesis and metastasis and improve anti-cancer immunotherapy.
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