Production and characterisation of a viral RNA-dependent RNA polymerase induced in Escherichia coli Q. 13 by infection with bacteriophage Q .

@article{Sargeant1971ProductionAC,
  title={Production and characterisation of a viral RNA-dependent RNA polymerase induced in Escherichia coli Q. 13 by infection with bacteriophage Q .},
  author={Ken Sargeant and R. B. G. Yeo and T G Vickers},
  journal={Biochimica et biophysica acta},
  year={1971},
  volume={238 3},
  pages={
          475-82
        }
}
Abstract 1. A simple method for producing large quantities of cells of Escherichia coli Q.13, rich in Qβ-RNA-dependent RNA polymerase is described. 2. Cultures of Escherichia coli Q.13 were grown at 37° in a 20-l stirred vessel having a sulphite oxidation capacity of about 220 mmoles O 2 /l per h. When the cell density had reached about 10 10 /ml, they were infected with bacteriophage Qβ using input ratios in the range 1.3–13.0 p.f.u./cell. Growth conditions were maintained for a further 35 min… CONTINUE READING