• Corpus ID: 22967948

Production and Characterization of Polyclonal Antibodies Specific for Entamoeba histolytica

  title={Production and Characterization of Polyclonal Antibodies Specific for Entamoeba histolytica},
  author={I. Canoy and Windell L. Rivera},
Murine polyclonal antibodies (pAbs) were produced by immunizing BALB/c mice with trophozoites of Entamoeba histolytica HK9 in Freund’s adjuvant. Using indirect fluorescent antibody test (IFAT), we have developed polyclonal IgG antibodies that specifically reacted with formalin-fixed E. histolytica trophozoites, but failed to bind with live trophozoites, suggesting the epitope to be cytoplasmic and not cell surface located. Entamoeba dispar, Entamoeba coli, and Blastocystis hominis showed no… 

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