• Corpus ID: 22967948

Production and Characterization of Polyclonal Antibodies Specific for Entamoeba histolytica

@inproceedings{Canoy2008ProductionAC,
  title={Production and Characterization of Polyclonal Antibodies Specific for Entamoeba histolytica},
  author={I. Canoy and Windell L. Rivera},
  year={2008}
}
Murine polyclonal antibodies (pAbs) were produced by immunizing BALB/c mice with trophozoites of Entamoeba histolytica HK9 in Freund’s adjuvant. Using indirect fluorescent antibody test (IFAT), we have developed polyclonal IgG antibodies that specifically reacted with formalin-fixed E. histolytica trophozoites, but failed to bind with live trophozoites, suggesting the epitope to be cytoplasmic and not cell surface located. Entamoeba dispar, Entamoeba coli, and Blastocystis hominis showed no… 

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SHOWING 1-10 OF 20 REFERENCES
Differentiation of Entamoeba histolytica from E. dispar facilitated by monoclonal antibodies against a 150-kDa surface antigen
TLDR
Results indicate that common antigenic epitopes of E. histolytica are on the 150-kDa surface molecule and that mAbs can distinguish between E. historicica and E. dispar.
Reactivity of monoclonal antibodies to species-specific antigens of Entamoeba histolytica.
TLDR
The combined use of monoclonal antibodies seems capable of distinguishing E. histolytica and/or E. history-like Laredo from other enteric protozoa.
Development of monoclonal antibodies specifically recognizing the cyst stage of Entamoeba histolytica.
TLDR
Three monoclonal antibodies were identified that reacted only with cysts and trophozoites of E. histolytica and can be used for differentiation and identification of E.'s histolyTica in feces.
Production and Characterization of Monoclonal Antibodies Against a Highly Immunogenic Fraction of Entamoeba histolytica (NIH:200) and Their Application in the Detection of Current Amoebic Infection
TLDR
A polyclonal‐monoclonal antibody‐based enzyme‐linked immunosorbent assay was developed for the detection of E. histolytica antigens in stool samples of infected patients and was found to be specific and sensitive and yielded 100% positive results in cases with amoebiasis.
A monoclonal antibody specific for a 40 kDa polypeptide recognizes pathogenic strains of Entamoeba histolytica.
In vitro interaction of Entamoeba histolytica trophozoites with collagen induces the intracellular formation and release of electron-dense granules (EDGs). We determined that four polypeptides in
A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica
TLDR
The production of a monoclonal antibody against E. histolytica is reported and its use in an immunofluorescence assay to identify invasive isolates cultured from stool samples of infected patients in several regions where amebiasis is endemic: Bangladesh, Colombia, and Mexico.
Identification of a pathogenic isolate-specific 30,000-Mr antigen of Entamoeba histolytica by using a monoclonal antibody
TLDR
The results suggest that the 30,000-molecular-weight antigen is a marker of pathogenic isolates and that the indirect fluorescent-antibody test with the MAb is useful for the accurate discrimination ofpathogenic amebae.
Development of Monoclonal Antibodies Which Specifically RecognizeEntamoeba histolytica in Preserved Stool Samples
ABSTRACT We report the generation of monoclonal antibodies against a recombinant 170-kDa subunit of the Gal or GalNAc lectin ofEntamoeba histolytica that specifically recognize E. histolytica but not
Diagnosis of amebic dysentery by detection of Entamoeba histolytica fecal antigen by an invasive strain-specific, monoclonal antibody-based enzyme-linked immunosorbent assay
TLDR
The FAC-ELISA is a potential alternative for the field diagnosis of amebic dysentery and for epidemiological studies to define the distribution of invasive E. histolytica.
The plasma membrane of Entamoeba histolytica: structure and dynamics *
TLDR
A comparative analysis of plasma membrane proteins of E histolytica trophozoites using three different isolation methods revealed that it is possible to select for specific membrane proteins, depending on the lysis conditions.
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