Production and Characterization of N- and C-terminally Truncated Mtx2: a Mosquitocidal Toxin from Bacillus sphaericus

@article{Phannachet2010ProductionAC,
  title={Production and Characterization of N- and C-terminally Truncated Mtx2: a Mosquitocidal Toxin from Bacillus sphaericus},
  author={Kulwadee Phannachet and Ponlawoot Raksat and Thidarat Limvuttegrijeerat and Boonhiang Promdonkoy},
  journal={Current Microbiology},
  year={2010},
  volume={61},
  pages={549-553}
}
Mosquitocidal toxin 2 (Mtx2) is a mosquito-larvicidal protein produced during vegetative stage of Bacillus sphaericus. The toxin consists of 292 amino acids and has a molecular weight of 31.8 kDa. To determine the active core region of the toxin, amino acids at N- and C-termini were sequentially removed. Deletion up to 23 amino acids from the N-terminus (Met1-Tyr23) did not significantly affect protein production and the toxin activity, whereas removal of 26 amino acids from the N-terminus… CONTINUE READING
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