Production, purification, and characterization of botulinolysin, a thiol-activated hemolysin of Clostridium botulinum

@article{Haque1992ProductionPA,
  title={Production, purification, and characterization of botulinolysin, a thiol-activated hemolysin of Clostridium botulinum},
  author={A Haque and Nakaba Sugimoto and Yasuhiko Horiguchi and Toshio Okabe and T Miyata and Sadaaki Iwanaga and Morihiro Matsuda},
  journal={Infection and Immunity},
  year={1992},
  volume={60},
  pages={71 - 78}
}
A hemolysin, botulinolysin, produced by Clostridium botulinum was purified to homogeneity and characterized. First, a strain of C. botulinum type C, strain C-203 Tox, which produced a large amount of hemolysin, was selected, and optimal culture medium and conditions for its production of hemolysin were determined. The hemolysin produced in the culture supernatant of this strain under optimal conditions was purified by a combination of ammonium sulfate precipitation, DEAE-Sepharose CL-6B column… 
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