Product of per locus of Drosophila shares homology with proteoglycans

@article{Jackson1986ProductOP,
  title={Product of per locus of Drosophila shares homology with proteoglycans},
  author={F Rob Jackson and Thaddeus A. Bargiello and Suk-Hyeon Yun and Michael W. Young},
  journal={Nature},
  year={1986},
  volume={320},
  pages={185-188}
}
Genes controlling biological rhythms have been identified in Drosophila1,2. The best characterized of these genes is called period (per). Although wild-type flies have daily (circadian) rhythms with a periodicity of ∼24 h, pers and perl mutants have 19-h and 29-h rhythms, respectively, and per0 mutants are arrhythmic1. The pers mutation also enhances the sensitivity of the circadian clock to resetting by light stimuli3, and all three types of per mutations affect a much shorter period ultradian… 
Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
TLDR
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks.
The Neurospora clock gene frequency shares a sequence element with the Drosophila clock gene period
TLDR
The cloning and analysis of the frq locus are reported and show it to be larger and more complex than would have been predicted from the available genetic data, and suggests the possibility of a common element in the clock mechanisms of these two organisms.
The period gene encodes a predominantly nuclear protein in adult Drosophila
TLDR
The results of an immunoelectron microscopic analysis of wild-type flies and per-beta- galactosidase (beta-gal) fusion gene transgenics using a polyclonal anti-PER antibody or an anti- beta-gal antibody suggest that PER acts in that subcellular compartment to affect circadian rhythms.
The clock gene period in the housefly, Musca domestica : a molecular analysis
TLDR
A comparative study suggests that the current model based on the negative feedback loop may be inadequate to explain the molecular mechanism underlying the circadian clock.
Genetics and molecular biology of rhythms
  • J. C. Hall, M. Rosbash
  • Biology, Medicine
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1987
TLDR
Nucleotide‐sequencing analyses of genomic DNA (and/or cDNA) from normal and mutated per alleles have led to the suggestion that this clock gene encodes a family of proteoglycans, and shown that the three types of per mutations are associated with interesting amino‐acid substitutions or a stop codon, respectively.
The molecular ethology of theperiod gene inDrosophila
TLDR
A model has been proposed, which suggests that the period (per) gene in Drosophila may determine periodicity by modulating intercellular communication.
The period clock locus of D. melanogaster codes for a proteoglycan
TLDR
To determine whether the per transcript codes for a proteoglycan, a region of its coding sequence was expressed (in bacteria) as part of a fusion protein, which was used to immunize rabbits and detected an antigen that is present in wild-type flies and absent in a per- mutant.
Molecular and behavioral analysis of four period mutants in Drosophila melanogaster encompassing extreme short, novel long, and unorthodox arrhythmic types.
TLDR
Three older per mutants-perT, perClk, and per04-along with a novel long-period one (perSLIH) are molecularly characterized and two mutations lie within regions of PER that have not been studied previously and may define important functional domains of this clock protein.
The molecular control of circadian behavioral rhythms and their entrainment in Drosophila.
  • M. W. Young
  • Medicine, Biology
    Annual review of biochemistry
  • 1998
TLDR
The recent discovery of PER homologues in mice and humans suggests that a related mechanism controls mammalian circadian behavioral rhythms.
Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 or
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It is reported that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored.
An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates
TLDR
The results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates show an unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologously to DNA in chicken, mouse and man.
Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms
TLDR
Evidence is discussed, from previously reported genetic and phenotypic analysis of per's function, suggesting that this region may be complex and that several gene products from the per region, including this 0.9 kb transcript, may be involved in the different aspects of normal rhythmicity influenced by this clock gene.
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Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment.
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Both circadian rhythms and a very short, noncircadian oscillation appear to be influenced by the same gene.
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Certain subsegments of the per region, transduced into the genome of arrhythmic pero flies, restore rhythmicity in circadian locomotor behavior and the male's courtship song.
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