Processivity, synergism, and substrate specificity of Thermobifida fusca Cel6B.

@article{Vuong2009ProcessivitySA,
  title={Processivity, synergism, and substrate specificity of Thermobifida fusca Cel6B.},
  author={Thu V Vuong and David B. Wilson},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 21},
  pages={6655-61}
}
A relationship between processivity and synergism has not been reported for cellulases, although both characteristics are very important for hydrolysis of insoluble substrates. Mutation of two residues located in the active site tunnel of Thermobifida fusca exocellulase Cel6B increased processivity on filter paper. Surprisingly, mixtures of the Cel6B mutant enzymes and T. fusca endocellulase Cel5A did not show increased synergism or processivity, and the mutant enzyme which had the highest… CONTINUE READING