Processiveness of DNA polymerases. A comparative study using a simple procedure.

@article{Das1979ProcessivenessOD,
  title={Processiveness of DNA polymerases. A comparative study using a simple procedure.},
  author={Salil K. Das and Robert K. Fujimura},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 4},
  pages={1227-32}
}
In this communication, we describe a simple procedure for analyzing the processiveness of DNA polymerases in general. By choosing conditions for which the number of incorporations per available primer is less than 1, we have reduced the probability of a primer molecule being utilized by the enzyme more than once. The primer-template used was poly(dA)300:oligo(dT)10, and the product was isolated by oligo(dT)-cellulose chromatography. The number of dTMP residues added per association was… CONTINUE READING

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