Processing of gene expression data generated by quantitative real-time RT-PCR.

@article{Muller2002ProcessingOG,
  title={Processing of gene expression data generated by quantitative real-time RT-PCR.},
  author={Patrick Y. Muller and Harald Janovjak and Andr{\'e} R. Miserez and Zuzana Dobbie},
  journal={BioTechniques},
  year={2002},
  volume={32 6},
  pages={1372-4, 1376, 1378-9}
}
Quantitative real-time PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistical analysis of the enormous amount of data gained by this technology, as these functions are not… CONTINUE READING
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