Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors.

@article{Shao1998ProcessingOD,
  title={Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors.},
  author={Z Shao and Yunhai Cui and Xiang Liu and Hong Yi and Jian-guo Ji and Zheyong Yu},
  journal={Journal of invertebrate pathology},
  year={1998},
  volume={72 1},
  pages={73-81}
}
Bombyx mori was found to be more sensitive to the protoxins of HD-1 than Heliothis armigera. SDS-PAGE analysis showed that a large amount of activated toxin was yielded from protoxin by B. mori gut juice while little was yielded by H. armigera. Further degradation of activated toxin was observed in H. armigera midgut juice detected by SDS-PAGE. pH influenced the proteolytic activity of the midgut juice significantly, but there was no obvious effect of pH on the degradation of activated toxin… CONTINUE READING