Process modeling of in situ-adsorption of a bacterial lipase.

  title={Process modeling of in situ-adsorption of a bacterial lipase.},
  author={Marcus Millitzer and Edda Wenzig and Wolfgang Peukert},
  journal={Biotechnology and bioengineering},
  volume={92 6},
In situ adsorption, known as an in situ-roduct removal (ISPR) technique for low molecular mass bioproducts, was in this study applied to a bacterial exoenzyme proving that this method is also suitable for the separation of macromolecules like proteins. For this, adsorbent particles were added to growing cultures of Staphylococcus carnosus rec., therefore both production and adsorption occurred simultaneously in shaking flasks, stirred tank, or airlift bioreactor as the chosen types of… CONTINUE READING