• Corpus ID: 67832009

Process for purification of high molecular weight hyaluronic acid

  title={Process for purification of high molecular weight hyaluronic acid},
  • Published 2012
  • Biology
(72) Inventors: • Santosh, Yvas Navi Mumbai 400 701 Maharashtra (IN) • Dharmendra, Jain Navi Mumbai 400 701 Maharashtra (IN) • Nataraj, Vedapuri Navi Mumbai 400 701 Maharashtra (IN) • Velankar, Harshad Navi Mumbai 400 701 Maharashtra (IN) • Kapat, Arnub Navi Mumbai 400 701 Maharashtra (IN) • Rangaswamy, Vidhya Navi Mumbai 400 701 Maharashtra (IN) (74) Representative: Srinivasan, Ravi Chandran J A Kemp 14 South Square Gray’s Inn London WC1R 5JJ (GB) 

Techno-Economic Analysis of a Hyaluronic Acid Production Process Utilizing Streptococcal Fermentation

A techno-economic analysis of a process for producing HA using Streptococcus zooepidemicus, simulated in SuperPro Designer, results in higher capital and operating costs, but also in higher profits, because HA for injectable use has a higher selling price that more than compensates for its higher production costs.

Effect of Various Physical Parameters and Statistical Medium Optimization on Production of Hyaluronic Acid Using S. Equi Subsp. Zooepidemicus ATCC 39920

It has been shown that initial conditions for bacterial cultivation are extremely important for the successful production of hyaluronic acid (HA) by fermentation. We investigated several physical

L'acide hyaluronique : Production microbiologique et applications

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Conception et optimisation d'un procédé extrapolable de purification d'acides hyaluroniques produits par culture microbienne

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Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography

Four size exclusion chromatography calibration techniques were tested for use in the molecular weight characterisation of Streptococcal Hyaluronic Acid and an exponential equation, evaluated using the Hamielec method, provided the most accurate estimates.

Culture Conditions Affect the Molecular Weight Properties of Hyaluronic Acid Produced by Streptococcus zooepidemicus

Replicate experiments performed under conditions resulting in the lowest (40(deg)C, anaerobic) and highest (40 g of glucose per liter, 1-vol/vol/min aeration)-M(infw) polymer demonstrated excellent experimental reproducibility.

Microbial synthesis of hyaluronan and chitin: New approaches.

To meet ever-increasing public demand, novel systems that can yield sufficient amounts of high-quality of HA and related materials are required.

Stable production of hyaluronic acid in Streptococcus zooepidemicus chemostats operated at high dilution rate.

The successful establishment of continuous culture at high dilution rate enables both commercial production at reduced cost and a more rational characterization and optimization of hyaluronic acid production in streptococci.

Molecular mechanisms and genetics of hyaluronan biosynthesis.

Novel and established applications of microbial polysaccharides.

Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus

SummaryThe optimum pH for both the rate of production and yield of hyaluronic acid (HA) byStreptococcus zooepidemicus from glucose medium was 6.7±0.2 under anaerobic conditions. High agitation rates

Synthesis and release of hyaluronic acid by Swiss 3 T 3 fibroblasts

The amount of cell-associated HA ofvarious cell populations correlated strongly with the synthesis of Hyaluronic acid in intact Swiss 3T3 mouse fibroblasts and isolated membranes.

Structured Model‐Based Analysis and Control of the Hyaluronic Acid Fermentation by Streptococcuszooepidemicus: Physiological Implications of Glucose and Complex‐Nitrogen‐Limited Growth

The hyaluronic acid (HA) fermentation by Streptococcus zooepidemicus under anaerobic and aerated conditions in glucose‐complex media was well described by a structured, two‐compartment model, and nitrogen‐limited growth was found to increase the HA to biomass yield, like that observed under glucose‐ limited growth, and the resulting HA molecular weight was reduced.