Procedures for the preparation and culture of 'reconstructed' rainbow trout branchial epithelia.

@article{Kelly2000ProceduresFT,
  title={Procedures for the preparation and culture of 'reconstructed' rainbow trout branchial epithelia.},
  author={S. Kelly and M. Fletcher and P. P{\"a}rt and C. Wood},
  journal={Methods in cell science : an official journal of the Society for In Vitro Biology},
  year={2000},
  volume={22 2-3},
  pages={
          153-63
        }
}
  • S. Kelly, M. Fletcher, +1 author C. Wood
  • Published 2000
  • Biology, Medicine
  • Methods in cell science : an official journal of the Society for In Vitro Biology
  • Techniques for the in vitro 'reconstruction' of freshwater rainbow trout branchial epithelia using the primary culture of gill cells on permeable polyethylene terephthalate cell culture filter supports are described. Representing models of the freshwater fish gill, epithelia grown by two separate techniques are composed of branchial pavement cells with or without the inclusion of mitochondria-rich (MR) cells. The generation of epithelia consisting of pavement cells only (via a method called… CONTINUE READING
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    References

    SHOWING 1-10 OF 45 REFERENCES
    Cultured branchial epithelia from freshwater fish gills
    • Wood, PÄRt
    • Biology, Medicine
    • The Journal of experimental biology
    • 1997
    • 122
    • PDF
    Passive and active transport properties of a gill model, the cultured branchial epithelium of the freshwater rainbow trout (Oncorhynchus mykiss).
    • C. Wood, K. Gilmour, P. Pärt
    • Medicine, Biology
    • Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
    • 1998
    • 66
    • PDF
    Fish Gill Respiratory Cells in Culture: A New Model for Cl−-secreting Epithelia
    • 62
    Primary culture of flounder renal tubule cells: transepithelial transport.
    • 42