Procedures for the preparation and culture of 'reconstructed' rainbow trout branchial epithelia.

@article{Kelly2000ProceduresFT,
  title={Procedures for the preparation and culture of 'reconstructed' rainbow trout branchial epithelia.},
  author={Scott P Kelly and Robert H. Fletcher and Peter P{\"a}rt and Chris M Wood},
  journal={Methods in cell science : an official journal of the Society for In Vitro Biology},
  year={2000},
  volume={22 2-3},
  pages={
          153-63
        }
}
Techniques for the in vitro 'reconstruction' of freshwater rainbow trout branchial epithelia using the primary culture of gill cells on permeable polyethylene terephthalate cell culture filter supports are described. Representing models of the freshwater fish gill, epithelia grown by two separate techniques are composed of branchial pavement cells with or without the inclusion of mitochondria-rich (MR) cells. The generation of epithelia consisting of pavement cells only (via a method called… CONTINUE READING

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