Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function.

Abstract

Caspase-3 is an effector caspase that is activated downstream of mitochondrial outer-membrane permeabilization (MOMP) during apoptosis. However, previous work has demonstrated that caspase-3-deficient mouse embryonic fibroblasts (MEFs) are resistant to mitochondrially mediated cell death and display a delay in the mitochondrial events of apoptosis, including Bax activation, MOMP and release of cytochrome c. Here, we show that caspase-3 regulates fibronectin secretion and impacts on cell morphology, adhesion and migration. Surprisingly, the catalytic activity of caspase-3 is not required for these non-apoptotic functions. Moreover, we found that caspase-3-deficient MEFs are not resistant to death by anoikis and that exogenous fibronectin protects wild-type MEFs from cell death induced by serum withdrawal. Taken together, our data indicate that procaspase-3 has a non-apoptotic function; it regulates the secretion of fibronectin and influences morphology, adhesion and migration. Furthermore, this novel procaspase-3 function might alter the apoptotic threshold of the cell.

DOI: 10.1242/jcs.135137

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Cite this paper

@article{Brentnall2014Procaspase3RF, title={Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function.}, author={Matthew Brentnall and David B. Weir and Anthony Rongvaux and Adam I. Marcus and Lawrence H. Boise}, journal={Journal of cell science}, year={2014}, volume={127 Pt 10}, pages={2217-26} }