Proboscidean DNA from Museum and Fossil Specimens: An Assessment of Ancient DNA Extraction and Amplification Techniques

  title={Proboscidean DNA from Museum and Fossil Specimens: An Assessment of Ancient DNA Extraction and Amplification Techniques},
  author={Hong Yang and Edward M Golenberg and Jeheskel Shoshani},
  journal={Biochemical Genetics},
Applications of reliable DNA extraction and amplification techniques to postmortem samples are critical to ancient DNA research. Commonly used methods for isolating DNA from ancient material were tested and compared using both soft tissue and bones from fossil and contemporary museum proboscideans. DNAs isolated using three principal methods served as templates in subsequent PCR amplifications, and the PCR products were directly sequenced. Authentication of the ancient origin of obtained… 

Ancient DNA analyses of museum specimens from selected Presbytis (primate: Colobinae) based on partial Cyt b sequences

The IUCN Red List of Threatened Species has categorized Malaysian primates from being data deficient to critically endanger. Thus, ancient DNA analyses hold great potential to understand phylogeny,

Successful PCR using Old Conservation Museum Fluid is Possible - Preliminary Studies on the ABO Group Model

Museum specimens are indubitably a valuable resource for studying diverse histological processes, but they are difficult to analyze because they typically contain short fragments of denatured and often degraded DNA.

Molecular Characterization of a Parasitic Tapeworm (Ligula) Based on DNA Sequences from Formalin-Fixed Specimens

It is demonstrated that endogenous DNA from specimens that were subjected to permanent formalin fixation can be routinely amplified from parasitic tapeworms, suggesting that fixation time in formalin may not be a critical factor affecting DNA degradation in such museum specimens.

Extraction and amplification of DNA from aged and archaeological Populus euphratica wood for species identification

Abstract The wood samples of Populus euphratica Oliv. (Salicaceae) are common archaeological plant remains in the hot and arid regions of western China. However, it is difficult to identify P.

Ancient DNA

The possibilities are considered of using ancient DNA in resolving issues of systematics and evolution of various animal taxa, population genetics of humans and rare species, taxonomic identification and paleontological reconstructions, geographic origin of populations, microbiological analysis of Paleontological and archeological finds, as well as some humanitarian aspects of its use.

A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections

A simple protocol for extracting and amplifying DNA segments from sloth museum specimens is presented, useful for genetic diversity studies of three-toed sloths and could be applied to other animals.

Accelerating plant DNA barcode reference library construction using herbarium specimens: improved experimental techniques

This study demonstrates that it is likely to build well‐covered reference libraries for DNA barcoding of vascular species in China using the reconstructed (self‐primed PCR amplified) DNA from the herbarium specimens.

New ancient DNA sequences suggest high genetic diversity for the woolly mammoth (Mammuthus primigenius)

It is suggested that a high intra-specific diversity existed in Mammuthus primigenius crossing both spatial and temporal ranges, resulting in a complex and divergent genetic background for DNA sequences so far recovered.

Ancient DNA: Recovery and Analysis

Ancient deoxyribonucleic acid (DNA) can be recovered from any preserved organic remains of both dead and living organisms and is characterized by degradation down to a few hundred base pairs. The



A blind testing design for authenticating ancient DNA sequences.

The results show that such a blind testing design performed in one laboratory, when coupled with phylogenetic analysis, can nonarbitrarily test the consistency and reliability of ancient DNA results, and can increase confidence in the authenticity of ancient sequences obtained from postmortem specimens.

Rapid isolation of DNA from fossil and museum specimens suitable for PCR.

It appears, at least in the authors' hands, that the procedure described here is a rapid and efficient way of obtaining small amounts of DNA for PCR in museum and fossilized specimens.

DNA extraction from Pleistocene bones by a silica-based purification method.

The following method, which is a modification of a protocol published by Boom et a/.

Improved efficiency in amplification of ancient DNA and its sequence analysis

It is suggested that in DNA samples from ancient sources a first series of ten cycles with a regular primer concentration (only 15 20 pM for few ancient targets), regular dNTP content (200 ktM; Boehringer), and regular, buffered reaction mix (10 mM Tris-HCl pH 8.3) is run under full stringency conditions to enhance PCR jumping.

DNA from Museum Specimens

A range of genetic material evolving at rates fast enough to distinguish between individuals, and slow enough to examine large scale systematic relationships, has become the latest tool for biological investigation of museum collections.

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

  • S. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
The polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance and opens up the prospect of performing diachronical studies of molecular evolutionary genetics.

Molecular phylogenetic inference from saber-toothed cat fossils of Rancho La Brea.

Comparison of fossil-derived DNA sequences to homologous regions in 15 living carnivorous species, including 9 species of Felidae and 6 nonfelids, affirmed the phylogenetic placement of Smilodon within the modern radiation ofFelidae distinct from the Miocene paleofelid (Nimravidae) saber-toothed "cat" species.

Mitochondrial DNA sequences from a 7000-year old brain.

The sequences show that this ancient individual belonged to a mitochondrial lineage that is rare in the Old World and not previously known to exist among Native Americans, bringing to three the number of maternal lineages known to have been involved in the prehistoric colonization of the New World.

Molecular genetic analyses of the Tyrolean Ice Man.

One DNA sequence of a hypervariable segment of the mitochondrial control region was determined independently in two different laboratories from internal samples of the body and showed that the mitochondrial type of the Ice Man fits into the genetic variation of contemporary Europeans and that it was most closely related to mitochondrial types determined from central and northern European populations.

Mitochondrial DNA from Ancient Bones

The discovery that DNA can also be recovered from ancient hones has created new possibilities for the study of past populations, as bones are abundant archaeological remains and many museums throughout the world contain extensive and well-characterized osteological collections.