Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher

@inproceedings{Normanno2015ProbingTT,
  title={Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher},
  author={Davide Normanno and Lydia Boudar{\`e}ne and Claire Dugast-Darzacq and Jiji Chen and Christian Richter and Florence Proux and Olivier B{\'e}nichou and Rapha{\"e}l Voituriez and Xavier Darzacq and Maxime Dahan},
  booktitle={Nature communications},
  year={2015}
}
Many cellular functions rely on DNA-binding proteins finding and associating to specific sites in the genome. Yet the mechanisms underlying the target search remain poorly understood, especially in the case of the highly organized mammalian cell nucleus. Using as a model Tet repressors (TetRs) searching for a multi-array locus, we quantitatively analyse the search process in human cells with single-molecule tracking and single-cell protein-DNA association measurements. We find that TetRs… CONTINUE READING
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