Probing the protein interaction network of Pseudomonas aeruginosa cells by chemical cross-linking mass spectrometry.

@article{Navare2015ProbingTP,
  title={Probing the protein interaction network of Pseudomonas aeruginosa cells by chemical cross-linking mass spectrometry.},
  author={Arti T. Navare and Juan D. Chavez and Chunxiang Zheng and Chad R. Weisbrod and Jimmy K. Eng and Richard J. Siehnel and Pradeep K. Singh and Colin S Manoil and James E. Bruce},
  journal={Structure},
  year={2015},
  volume={23 4},
  pages={762-73}
}
In pathogenic Gram-negative bacteria, interactions among membrane proteins are key mediators of host cell attachment, invasion, pathogenesis, and antibiotic resistance. Membrane protein interactions are highly dependent upon local properties and environment, warranting direct measurements on native protein complex structures as they exist in cells. Here we apply in vivo chemical cross-linking mass spectrometry, to reveal the first large-scale protein interaction network in Pseudomonas… CONTINUE READING