Probing the microenvironment of sol-gel entrapped cutinase: the role of added zeolite NaY.

@article{Vidinha2008ProbingTM,
  title={Probing the microenvironment of sol-gel entrapped cutinase: the role of added zeolite NaY.},
  author={P. Vidinha and V. Augusto and J. Nunes and J. Lima and J. Cabral and Susana Barreiros},
  journal={Journal of biotechnology},
  year={2008},
  volume={135 2},
  pages={
          181-9
        }
}
  • P. Vidinha, V. Augusto, +3 authors Susana Barreiros
  • Published 2008
  • Chemistry, Medicine
  • Journal of biotechnology
  • Cutinase, an esterase from Fusarium solani pisi, was immobilized in sol-gel matrices of composition 1:5 tetramethoxysilane (TMOS):n-alkyltrimethoxysilane (n-alkylTMS). Fluorescence spectroscopy using the single tryptophan (Trp-69) residue of cutinase as a probe revealed that the polarity of the matrices decreased as their hydrophobicity increased up to the TMOS/n-butylTMS pair, which correlates with an increase in cutinase activity. Fluorescence emission was suppressed (a higher than two orders… CONTINUE READING
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