Probing the membrane topology of Candida tropicalis cytochrome P450.

@article{Sanglard1993ProbingTM,
  title={Probing the membrane topology of Candida tropicalis cytochrome P450.},
  author={Dominique Sanglard and C Sengstag and W Seghezzi},
  journal={European journal of biochemistry},
  year={1993},
  volume={216 2},
  pages={477-85}
}
The membrane topology of two alkane-inducible cytochromes P450 from the yeast Candida tropicalis, alk1 and alk2, was tested by construction of fusion proteins with part of invertase and histidinol dehydrogenase (invHIS4C) and expression in a Saccharomyces cerevisiae his4 mutant. Depending on the localization of invHIS4C on the endoplasmic reticulum (ER) cytoplasmic or luminal side, the enzyme converts histidinol to histidine and allows the his4 yeast strain to grow on histidinol-supplemented… CONTINUE READING