Probing the Architecture of a Simple Kinetochore Using DNA–Protein Crosslinking

@article{Espelin1997ProbingTA,
  title={Probing the Architecture of a Simple Kinetochore Using DNA–Protein Crosslinking},
  author={C. Espelin and K. Kaplan and P. Sorger},
  journal={The Journal of Cell Biology},
  year={1997},
  volume={139},
  pages={1383 - 1396}
}
  • C. Espelin, K. Kaplan, P. Sorger
  • Published 1997
  • Biology, Medicine
  • The Journal of Cell Biology
  • In budding yeast, accurate chromosome segregation requires that one and only one kinetochore assemble per chromosome. In this paper, we report the use of DNA–protein crosslinking and nondenaturing gel analysis to study the structure of CBF3, a four-protein complex that binds to the essential CDEIII region of Saccharomyces cerevisiae centromeres. We find that three subunits of CBF3 are in direct contact with CDEIII over a region of DNA that spans 80 bp. A highly asymmetric core complex… CONTINUE READING

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