Probing the Architecture of a Simple Kinetochore Using DNA–Protein Crosslinking

@article{Espelin1997ProbingTA,
  title={Probing the Architecture of a Simple Kinetochore Using DNA–Protein Crosslinking },
  author={Christopher W. Espelin and Kenneth B. Kaplan and Peter K. Sorger},
  journal={The Journal of Cell Biology},
  year={1997},
  volume={139},
  pages={1383 - 1396}
}
In budding yeast, accurate chromosome segregation requires that one and only one kinetochore assemble per chromosome. In this paper, we report the use of DNA-protein crosslinking and nondenaturing gel analysis to study the structure of CBF3, a four-protein complex that binds to the essential CDEIII region of Saccharomyces cerevisiae centromeres. We find that three subunits of CBF3 are in direct contact with CDEIII over a region of DNA that spans 80 bp. A highly asymmetric core complex… CONTINUE READING
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