Probing the ATP-binding site of P1 ParA: partition and repression have different requirements for ATP binding and hydrolysis.

@article{Fung2001ProbingTA,
  title={Probing the ATP-binding site of P1 ParA: partition and repression have different requirements for ATP binding and hydrolysis.},
  author={Eula L. Fung and Jean-Yves Bouet and Barbara E. Funnell},
  journal={The EMBO journal},
  year={2001},
  volume={20 17},
  pages={4901-11}
}
The ParA family of proteins is involved in partition of a variety of plasmid and bacterial chromosomes. P1 ParA plays two roles in partition: it acts as a repressor of the par operon and has an undefined yet indispensable role in P1 plasmid localization. We constructed seven mutations in three putative ATP-binding motifs of ParA. Three classes of phenotypes resulted, each represented by mutations in more than one motif. Three mutations created 'super-repressors', in which repressor activity was… CONTINUE READING