Probing structural stability of chromatin assembly sorted from living cells.

@article{Hameed2009ProbingSS,
  title={Probing structural stability of chromatin assembly sorted from living cells.},
  author={Feroz Meeran Hameed and Gautam V. Soni and Harini Krishnamurthy and G. V. Shivashankar},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={385 4},
  pages={518-22}
}
Post-translational modifications of the histone tails and other chromatin binding proteins affect the stability of chromatin structure. In this study, we have purified chromatin from live cell nuclei using a fluorescence activated cell sorter (FACS) and studied the structural stability of this self-assembled structure. Using total internal reflection fluorescence (TIRF) microscopy, we map the effect of covalent modifications on the interaction of histone-DNA complex, by measuring the… CONTINUE READING

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