Probing single-stranded DNA and its biomolecular interactions through direct catalytic activation of factor XII, a protease of the blood coagulation cascade.

Abstract

Triggered self-activation of factor XII, a blood coagulation protease, was utilized for the amplified visual detection of ss-DNA targets in a non-sequence specific way. Factor XII holds potential as a low-affinity and therefore non-interfering probe for DNA secondary structure and for the screening of protein binding to ss-DNA. The observation that ss-DNA also accelerates coagulation of human blood plasma is relevant to the emerging field of aptamer therapeutics.

Statistics

020040020072008200920102011201220132014201520162017
Citations per Year

136 Citations

Semantic Scholar estimates that this publication has 136 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Pavlov2006ProbingSD, title={Probing single-stranded DNA and its biomolecular interactions through direct catalytic activation of factor XII, a protease of the blood coagulation cascade.}, author={Valeri Pavlov and Michael Zorn and Roland Kraemer}, journal={Biochemical and biophysical research communications}, year={2006}, volume={349 3}, pages={1011-5} }