Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides

  title={Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides},
  author={E. Rapoport and G. Pazynina and M. Sablina and P. Crocker and N. Bovin},
  journal={Biochemistry (Moscow)},
  • E. Rapoport, G. Pazynina, +2 authors N. Bovin
  • Published 2006
  • Chemistry, Medicine
  • Biochemistry (Moscow)
  • Soluble siglecs-1,-4,-5,-6,-7,-8,-9, and-10 were probed with polyacrylamide glycoconjugates in which: 1) the Neu5Ac residue was substituted by a sulfate group (Su); 2) glycoconjugates contained both Su and Neu5Ac; 3) sialoglycoconjugates contained a tyrosine-O-sulfate residue. It was shown that sulfate derivatives of LacNAc did not bind siglecs-1,-4,-5,-6,-7,-8,-9, and-10; binding of 6′-O-Su-LacNAc to siglec-8 was stronger than binding of 3′SiaLacNAc. The relative affinity of 3′-O-Su-TF binding… CONTINUE READING
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