Probing of tertiary interactions in RNA: 2'-hydroxyl-base contacts between the RNase P RNA and pre-tRNA.

@article{Pan1995ProbingOT,
  title={Probing of tertiary interactions in RNA: 2'-hydroxyl-base contacts between the RNase P RNA and pre-tRNA.},
  author={Tao Pan and Andrea Loria and Kejun Zhong},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 26},
  pages={12510-4}
}
A general method has been developed to analyze all 2' hydroxyl groups involved in tertiary interactions in RNA in a single experiment. This method involves comparing the activity of populations of circularly permuted RNAs that contain or lack potential hydrogen-bond donors at each position. The 2' hydroxyls of the pre-tRNA substrate identified as potential hydrogen bond donors in intermolecular interactions with the ribozyme from eubacterial RNase P (P RNA) are located in the T stem and T loop… CONTINUE READING

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