Probing fast ribozyme reactions under biological conditions with rapid quench-flow kinetics.

Abstract

Reaction kinetics on the millisecond timescale pervade the protein and RNA fields. To study such reactions, investigators often perturb the system with abiological solution conditions or substrates in order to slow the rate to timescales accessible by hand mixing; however, such perturbations can change the rate-limiting step and obscure key folding and… (More)
DOI: 10.1016/j.ymeth.2017.03.011

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