Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions

  title={Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions},
  author={Cl{\'e}mence Roggo and Nicolas Carraro and Jan Roelof van der Meer},
  booktitle={Scientific Reports},
Bacterial chemotaxis signaling may be interesting for the development of rapid biosensor assays, but is difficult to quantify. Here we explore two potential fluorescent readouts of chemotactically active Escherichia coli cells. In the first, we probed interactions between the chemotaxis signaling proteins CheY and CheZ by fusing them individually with non-fluorescent parts of stable or unstable ‘split’-Green Fluorescent Protein. Wild-type chemotactic cells but not mutants lacking the CheA… CONTINUE READING
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