Probing biomembrane interfacial potential and pH profiles with a new type of float-like fluorophores positioned at varying distance from the membrane surface.

@article{Kraayenhof1993ProbingBI,
  title={Probing biomembrane interfacial potential and pH profiles with a new type of float-like fluorophores positioned at varying distance from the membrane surface.},
  author={Ruud Kraayenhof and Geert Sterk and Helen Sang},
  journal={Biochemistry},
  year={1993},
  volume={32 38},
  pages={10057-66}
}
Fluorophores of a new type were synthesized to probe the electrostatic potential or pH profiles in the external interface of biomembranes. The probes consist of the pH-sensitive fluorophore 7-hydroxycoumarin, coupled to a tetradecyl (myristyl) tail by a spacer group of varying length. A positively charged group is included between the tetradecyl and spacer groups to encourage a float-like alignment in the membrane head-group region. Three probes of this type were compared with 4-heptadecyl-7… CONTINUE READING

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