Probing bacterial nucleoid structure with optical tweezers.


The H-NS protein is a major component of the nucleoid in Gram-negative bacterial cells. It is a global regulator of transcription that affects the expression of many genes, including virulence genes in pathogenic species. At a local level, it facilitates the formation of nucleoprotein structures that repress transcriptional promoter function. H-NS can form bridges between different DNA molecules or between different sections of the same molecule, allowing it to compact and impose structure on the nucleoid. A recent paper by Dame et al.1 reports new insights into H-NS-mediated DNA bridging that were obtained using an optical tweezers device.

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@article{Dorman2007ProbingBN, title={Probing bacterial nucleoid structure with optical tweezers.}, author={Charles J. Dorman}, journal={BioEssays : news and reviews in molecular, cellular and developmental biology}, year={2007}, volume={29 3}, pages={212-6} }