Probing Protein-Protein Interactions Using Asymmetric Labeling and Carbonyl-Carbon Selective Heteronuclear NMR Spectroscopy

@inproceedings{Larsen2018ProbingPI,
  title={Probing Protein-Protein Interactions Using Asymmetric Labeling and Carbonyl-Carbon Selective Heteronuclear NMR Spectroscopy},
  author={Erik K. Larsen and Cristina Olivieri and Caitlin J Walker and S ManuV and Jiali Gao and David A Bernlohr and Mauro Tonelli and John L. Markley and Gianluigi Veglia},
  booktitle={Molecules},
  year={2018}
}
Protein-protein interactions (PPIs) regulate a plethora of cellular processes and NMR spectroscopy has been a leading technique for characterizing them at the atomic resolution. Technically, however, PPIs characterization has been challenging due to multiple samples required to characterize the hot spots at the protein interface. In this paper, we review our recently developed methods that greatly simplify PPI studies, which minimize the number of samples required to fully characterize residues… CONTINUE READING

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