Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.

@article{Immormino2015ProbingMS,
  title={Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.},
  author={Robert M. Immormino and Chrystal A. Starbird and Ruth E. Silversmith and Robert B. Bourret},
  journal={Biochemistry},
  year={2015},
  volume={54 22},
  pages={3514-27}
}
Response regulator signaling proteins and phosphatases of the haloacid dehalogenase (HAD) superfamily share strikingly similar folds, active site geometries, and reaction chemistry. Proteins from both families catalyze the transfer of a phosphoryl group from a substrate to one of their own aspartyl residues, and subsequent hydrolysis of the phosphoprotein. Notable differences include an additional Asp that functions as an acid/base catalyst and an active site well-structured prior to… CONTINUE READING