Probing DNA sequences in solution with a monomer-excimer fluorescence color change.

@article{Paris1998ProbingDS,
  title={Probing DNA sequences in solution with a monomer-excimer fluorescence color change.},
  author={Pamela L Paris and Joseph M. Langenhan and Eric T. Kool},
  journal={Nucleic acids research},
  year={1998},
  volume={26 16},
  pages={
          3789-93
        }
}
The use of a simple fluorescent nucleoside analogue in detection of point mutations by hybridization in solution is described. Pyrene is placed at 3' and 5' ends of a pair of oligodeoxynucleotide probes via a phosphoramidite derivative of deoxyribose with this fluorophore attached at the 1' position, replacing a DNA base. Adjacent binding of dual probes containing this fluorophore to a complementary target sequence results in a pronounced spectral change from blue pyrene monomer emission… CONTINUE READING

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