Pro region C-terminus:protease active site interactions are critical in catalyzing the folding of alpha-lytic protease.

@article{Peters1998ProRC,
  title={Pro region C-terminus:protease active site interactions are critical in catalyzing the folding of alpha-lytic protease.},
  author={Reuben J Peters and Andrew K. Shiau and Julie L. Sohl and David E. Anderson and G Tang and Joy L. Silen and David A. Agard},
  journal={Biochemistry},
  year={1998},
  volume={37 35},
  pages={12058-67}
}
alpha-Lytic protease is encoded with a large (166 amino acid) N-terminal pro region that is required transiently both in vivo and in vitro for the correct folding of the protease domain [Silen, J. L. , and Agard, D. A. (1989) Nature 341, 462-464; Baker, D., et al. (1992) Nature 356, 263-265]. The pro region also acts as a potent inhibitor of the mature enzyme [Baker, D., et al. (1992) Proteins: Struct.,Funct., Genet. 12, 339-344]. This inhibition is mediated through direct steric occlusion of… CONTINUE READING