Prions hijack tunnelling nanotubes for intercellular spread.

Abstract

In variant Creutzfeldt-Jakob disease, prions (PrP(Sc)) enter the body with contaminated foodstuffs and can spread from the intestinal entry site to the central nervous system (CNS) by intercellular transfer from the lymphoid system to the peripheral nervous system (PNS). Although several means and different cell types have been proposed to have a role, the mechanism of cell-to-cell spreading remains elusive. Tunnelling nanotubes (TNTs) have been identified between cells, both in vitro and in vivo, and may represent a conserved means of cell-to-cell communication. Here we show that TNTs allow transfer of exogenous and endogenous PrP(Sc) between infected and naive neuronal CAD cells. Significantly, transfer of endogenous PrP(Sc) aggregates was detected exclusively when cells chronically infected with the 139A mouse prion strain were connected to mouse CAD cells by means of TNTs, identifying TNTs as an efficient route for PrP(Sc) spreading in neuronal cells. In addition, we detected the transfer of labelled PrP(Sc) from bone marrow-derived dendritic cells to primary neurons connected through TNTs. Because dendritic cells can interact with peripheral neurons in lymphoid organs, TNT-mediated intercellular transfer would allow neurons to transport prions retrogradely to the CNS. We therefore propose that TNTs are involved in the spreading of PrP(Sc) within neurons in the CNS and from the peripheral site of entry to the PNS by neuroimmune interactions with dendritic cells.

DOI: 10.1038/ncb1841
0200400600800200920102011201220132014201520162017
Citations per Year

3,696 Citations

Semantic Scholar estimates that this publication has 3,696 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Gousset2009PrionsHT, title={Prions hijack tunnelling nanotubes for intercellular spread.}, author={Karine Gousset and Edwin Schiff and Christelle Langevin and Zrinka Marijanovic and Anna Maria Caputo and Duncan T Browman and Nicolas Chenouard and Fabrice de Chaumont and Angelo De Martino and Jost Enninga and Jean-Christophe Olivo-Marin and Daniela N M{\"a}nnel and Chiara Zurzolo}, journal={Nature cell biology}, year={2009}, volume={11 3}, pages={328-36} }