Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience

@article{Svoboda2006PrinciplesOT,
  title={Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience},
  author={Karel Svoboda and Ryohei Yasuda},
  journal={Neuron},
  year={2006},
  volume={50},
  pages={823-839}
}
The brain is complex and dynamic. The spatial scales of interest to the neurobiologist range from individual synapses (approximately 1 microm) to neural circuits (centimeters); the timescales range from the flickering of channels (less than a millisecond) to long-term memory (years). Remarkably, fluorescence microscopy has the potential to revolutionize research on all of these spatial and temporal scales. Two-photon excitation (2PE) laser scanning microscopy allows high-resolution and high… Expand
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