Principles and challenges of genome-wide DNA methylation analysis

  title={Principles and challenges of genome-wide DNA methylation analysis},
  author={Peter W. Laird},
  journal={Nature Reviews Genetics},
  • P. Laird
  • Published 2 February 2010
  • Biology
  • Nature Reviews Genetics
Methylation of cytosine bases in DNA provides a layer of epigenetic control in many eukaryotes that has important implications for normal biology and disease. Therefore, profiling DNA methylation across the genome is vital to understanding the influence of epigenetics. There has been a revolution in DNA methylation analysis technology over the past decade: analyses that previously were restricted to specific loci can now be performed on a genome-scale and entire methylomes can be characterized… 

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  • C. Bock
  • Biology
    Nature Reviews Genetics
  • 2012
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Recent identification of oxidation derivatives of cytosine methylation in mammalian DNA raises the possibility that DNA methylation patterns are more dynamic than previously anticipated.



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A genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion, which is more cost-effective than mCIP and requires as little as 100 ng of Arabidopsis DNA.

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